Can I document Blot In Gel documentation when using chemiluminescence ECL?

19 February 2015 1 9K Report

I do have ECL chemiluminescence for blotting. Finding difficult with x ray film developer.

Would it be possible to use Gel doc (Used for electrophoresis gel imaging) for blot imaging?

If it is difficult for you to get image from film, you can use DAB system to get your signal directly on the blot. You just need to change ECL system to DAB system. You can get more detail information from this website.

http://www.google.co.jp/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0CDQQFjAD&url=http%3A%2F%2Fwww.nacalai.co.jp%2Fglobal%2Fdownload%2Fpdf%2Fwestern_blot_protocols.pdf&ei=RozlVI--CaTZmgXkuIGYAw&usg=AFQjCNHIerT35PwWbkDY4u3DPV8KmhrA_g&sig2=Ip7ZTgsFO_PWjiN6oP3GUw&bvm=bv.85970519,d.dGY

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