i am doing experiment on brain homogenate from terminally dead syrian golden hamsters....i want to check Calcineurin level in homogenates...i will use Calbiochem cellular calcineurin activity kit....the kit instructions want me to centrifuge the homogenate at 100,000 g speed but i can't find ultracentrifuge machine in our laboratory...can i do low speed centrifugation? and how about maintaining 4 degree Celsius temprature? i will be thankful for any kind advise...
Regards...Ali