SPSS - how to create new variable include and excluding cases based on another variable?

Hi there, I have been searching and searching and cannot find an answer to this problem... I have a variable (Ref_Accepted_Comb) for the number of referrals accepted with values of (1=Yes, 2=No,...

08 February 2021 3,479 3 View

Does maternal potentiation work?

Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...

11 January 2021 558 2 View

Anybody know how can I analyze differences between groups vocalization syntax using the transition probability between syllables?

I have been using a MATLAB script called DeepSqueak to analyze vocal communication in mice. One of the outcomes of this analysis is the transition probability between the different syllables that...

09 December 2020 1,525 1 View

Anyone with experience conducting proximity ligation assay (PLA) in mouse heart tissue ?

I was wondering if anyone had any experience doing proximity ligation assays (PLA) using mouse whole heart (ventricular) tissue (in any state)?

29 November 2020 9,103 1 View

PCA: What is the significance/meaning of a smaller ellipse?

First, is there a test to be able to say an ellipse in a PCA plot is "smaller" than another? Second, what is the significance/meaning of a smaller ellipse? (Greater constriction/Less...

15 November 2020 2,234 4 View

How to extract/remove ethanol from kava tincture?

Producing Kava tincture using different alcohol concentrstion as solvent, soaked for 3 weeks filter off the kava paste and left with mixture of kavalactones and ethanol..need a method to remove...

14 June 2020 5,104 1 View

Can you have one variable load onto two factors in a CFA, prior to SEM?

I am running a SEM, and I have 6 variables which will load onto two factors (positive and negative mental health). However, this model does not meet a good model fit. When I allow depression to...

19 May 2020 8,286 3 View

When does someone use corrected fin length and the actual fin length in heat transfer problems?

In some instances the extended length of a fin is used but then in other instances the normal fin length is used in extended surface heat transfer problems.

23 March 2020 7,553 4 View

How will I calculate the surface temperature of a HDPE pipe that is exposed to the sun?

I need to calculate the final temperature of a pipe that's been lying in the sun for the day, however, I am unsure how to do this. I know how to calculate the heat transfer through the pipe wall...

19 January 2020 1,259 10 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

14 September 2019 3,577 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View