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Questions related from Danielle Amaral
I am planning to do some viability assays using oocytes from a marine animal. I did not found recommendations about trypan blue dilution since the commercial one is diluted in freshwater or PBS...
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My research group sequenced the genome of a plant with potential commercial interest. We want to publish the article, describing the genome sequence, completeness of our genome, assemble...
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I used nickel column to purify a protein with 50 kDa and 6-his-tag. If I load the column with 20mg of protein, is there an average yield I can expect? Or does it vary? After purification I tried...
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I have rotor gene sybr green for the Qiagen equipment, but it´s broken. I want to know if I can use this kit to perfome the qRT-PCR reations on Step One equipment, using 96 well plates.
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We sequenced 20 samples of 2 different regions. A total of 40 samples. We used different primer pairs aiming to recover a broad range of groups. However, PCR/sequencing failed for different...
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