Danielle Amaral

4 Questions 5 Answers 0 Followers

Questions related from Danielle Amaral

Can I dilute trypan blue in salted water?

I am planning to do some viability assays using oocytes from a marine animal. I did not found recommendations about trypan blue dilution since the commercial one is diluted in freshwater or PBS...

13 July 2020 7,368 0 View

How to keep my genome sequence private?

My research group sequenced the genome of a plant with potential commercial interest. We want to publish the article, describing the genome sequence, completeness of our genome, assemble...

13 September 2018 10,103 3 View

Is there a standard average yield for protein purification on nickel (GE)? How much protein can I expect after purification?

I used nickel column to purify a protein with 50 kDa and 6-his-tag. If I load the column with 20mg of protein, is there an average yield I can expect? Or does it vary? After purification I tried...

29 August 2017 5,188 7 View

Can I use rotor gene sybr green (Qiagen) on Step one (applied biosystems)?

I have rotor gene sybr green for the Qiagen equipment, but it´s broken. I want to know if I can use this kit to perfome the qRT-PCR reations on Step One equipment, using 96 well plates.

26 May 2017 3,333 2 View