I'm looking for gene expression of GA20OX in plant. However, no full sequence of that gene been identified in that plant, but only short fragment (from cDNA library) of that gene been published recently. So, I'm using the same primer that they used for cDNA library to check my gene expression in the same plant where i extract the RNA, synthesis cDNA, and run my PCR. And now I'm waiting for the sequencing result (expected to be 74bp only) to confirm it is the right gene. I'm not sure whether i'm doing the right thing using that primer to check the gene expression.

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