How compare between threshold value and mean gray grayscale image in python ?

Iam working on signature image and I need to find geometric features like(slant,pressure,size,baseline)

15 February 2021 7,565 2 View

Could two diffrent compounds have same FTIR spectrum but different NMR?

I extracted 2 compounds from plants one was oil the other White PPT but both of them has exactly the same ftir spectrum but different NMR spectrum any is their any suggestions plz

14 April 2018 2,456 4 View

How can i genotype Campylobacter using the sequence data from seven housekeeping genes ??

Hello to all, I'm doing a master thesis on Campylobacter jejuni and I'm down to the genotyping, I'm using MLST and I obtained the first products (900 bp) however, they need to reduced to 500 bp...

01 April 2018 908 7 View

How can I conduct a correlation test between a nominal variable and a scale or continuous variable?

hello everyone, How can I conduct a correlation test between a nominal variable (gender) and a scale or continuous variable (mean of productivity for the employee)? the mean of productivity is...

04 December 2016 5,707 20 View

Where can i find the WHI or the Work & Health Interview survey?

WHI is "The WHI is a computer-assisted telephone interview designed to quantify lost productive work time, including time absent from work and reduced performance while at work as a result of...

02 October 2016 5,263 1 View

Regulatory guidelines for new surfactants to be used in pharmaceutical applications, would you add relevant regulatory?

Hello, I have been searching for updated regulatory guidelines for new surfactants to be used in pharmaceutical applications (focusing on safety and efficacy aspects). I found the " Nonclinical...

01 January 1970 9,077 4 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View