I want my growth curve in short period. If I will add high inoculum, It will create any problem?
I have a spectrophotometer with temperature controller and capable for record reading each minute but some times this system getting hang after 8 hour and the number of cultures are large thats why I want my growth curve within 8 hour.
I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.
17 July 2024 5,633 4 View
Two independent variables 1. Attachment style 1 to 5 likert scale Strongly disagree to Strongly agree 2. Family functioning 1 to 4 likert scale Strongly agree to strongly disagree 3. Childhood...
10 February 2024 6,384 2 View
while using the TRIZOL RNA extraction method. In the precipitation step using isopropanol, polysaccharides like pectin and xyloglucan also get precipitated along with RNA, hindering the following...
30 January 2024 1,390 0 View
1. http://www.libraweb.net/riviste.php?chiave=114 2. http://tbf.peerjournals.net/ Which one is original
26 December 2023 2,961 1 View
Proteins expressed in pPcZaA for protein purification using his trap column in FPLC? how to prepare a sample for that. We have lyophilized the supernatant at 24 hr, 48 hr and run SDS.
25 December 2023 9,012 0 View
In our ongoing study we aim to assess the fecal microbiome and metabolome in patients in Indian population. For achieving the same we are referring to Franzosa Eric et al's., study entitled "Gut...
11 December 2023 8,754 0 View
please define steps how to upload research paper
27 November 2023 6,287 2 View
Hi everyone, We use DAMASK 3.0. We want to do dwell fatigue simulations. Can we use phenomenological power law for dwell fatigue/creep simulations? Which Model is best for dwell fatigue/creep...
17 October 2023 3,632 0 View
I am trying to validate the detection of plant fungal pathogen through LAMP reaction. But I have an issue with the LAMP reaction. I am detecting the band in NTC (Control) also. To rectify the...
10 October 2023 1,525 2 View
In exothermic reactions, the rate constant decreases on increasing the temperature. When we plot the graph between ln k and 1/T, a positive slope is obtained which gives negative activation...
21 August 2023 372 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View