while using the TRIZOL RNA extraction method. In the precipitation step using isopropanol, polysaccharides like pectin and xyloglucan also get precipitated along with RNA, hindering the following steps. Can anyone suggest any solution for this?
Two independent variables 1. Attachment style 1 to 5 likert scale Strongly disagree to Strongly agree 2. Family functioning 1 to 4 likert scale Strongly agree to strongly disagree 3. Childhood...
10 February 2024 6,384 2 View
1. http://www.libraweb.net/riviste.php?chiave=114 2. http://tbf.peerjournals.net/ Which one is original
26 December 2023 2,961 1 View
Proteins expressed in pPcZaA for protein purification using his trap column in FPLC? how to prepare a sample for that. We have lyophilized the supernatant at 24 hr, 48 hr and run SDS.
25 December 2023 9,012 0 View
please define steps how to upload research paper
27 November 2023 6,287 2 View
Hi everyone, We use DAMASK 3.0. We want to do dwell fatigue simulations. Can we use phenomenological power law for dwell fatigue/creep simulations? Which Model is best for dwell fatigue/creep...
17 October 2023 3,632 0 View
I am trying to validate the detection of plant fungal pathogen through LAMP reaction. But I have an issue with the LAMP reaction. I am detecting the band in NTC (Control) also. To rectify the...
10 October 2023 1,525 2 View
In exothermic reactions, the rate constant decreases on increasing the temperature. When we plot the graph between ln k and 1/T, a positive slope is obtained which gives negative activation...
21 August 2023 372 1 View
How can the Thermogravimetric analysis (TGA) be used to compare similar products?
19 August 2023 3,742 1 View
Could anyone tell how can the TGA (Thermogravimetric analysis) be used to reverse engineer a product?
19 August 2023 9,667 3 View
Can we prepare hydrazine hydrate buffer solution? Please provide reference.
02 August 2023 1,011 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View