while using the TRIZOL RNA extraction method. In the precipitation step using isopropanol, polysaccharides like pectin and xyloglucan also get precipitated along with RNA, hindering the following steps. Can anyone suggest any solution for this?
I am doing buckling analysis of laminated plate surface bounded with two piezoelectric PZT 5H and axial compressive load is applied. I am getting an error, No ANEL table exists for material 2....
16 February 2021 510 1 View
I am doing a meta analysis of imaging exploratory studies which themselves did not have effect size. Therefore, I am unable to compute the variability in the effect sizes in my meta analysis. What...
18 January 2021 4,881 4 View
I did find some papers that incorporate the two ideas together but I could not follow the research methodology. The simulation results were remarkable but not self explanatory. I tried to follow...
20 September 2020 5,354 4 View
Pigeonpea is a preferred host to adult blister beetle (Mylabris pustulata). Early pigeonpea, which is sown during May 15-June 15 in North West and north east plains of India, enters into flowering...
28 July 2020 1,278 8 View
In recent years, data science has emerged as a promising interdisciplinary subject, and helped understand and analyze actual phenomena with data in multiple areas. The availability and...
08 June 2020 6,514 6 View
Before the Corona pandemic, agriculture sector in India contributed around 15 percent to the total GDP growth. In the present scenario, service and industrial sectors are bound to pull down the...
27 April 2020 9,608 11 View
Cool season pulses such as chickpea, lentil, grass pea, faba bean, etc are affected by a number of root and foliar diseases. Empirical observation revealed that there was minimal incidence of root...
26 March 2020 724 26 View
I am working on the enhancement of heat transfer using nanofluids from the past few years. But sometimes, I think that the research in the applications of nanofluids is saturated. If not, what are...
27 February 2020 4,764 9 View
I am implementing InAs/GaSb superlattice mid-wavelength infrared pin photodiode structure in SILVACO ATLAS. I am not able to understand what does" ML" in device structure means and how can i do...
22 January 2020 9,283 6 View
In nanodusty plasma, dust density is higher than normal dusty plasma. In numerical modeling, it is assumed that it is electron depleted dusty plasma. They ignore the electron charge in compared to...
13 January 2020 3,925 5 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View