Can hydroxylamine.HCl be used as a chaotropic agent to improve protein solubility?

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Steingrimur Stefansson

Hi İsmail Emir Akyildiz . Not a good idea.

Urea/guanidine are chemically inert chaotropes that do not modify proteins.

Hydroxylamine:HCl?. Not so much.

Adron Ung

Amino acid L-Arginine is used to improve protein solubility. There is some literature about this. Typically arginine is used in the buffers at a concentration of 50 to 100 millimolar. Apparently it suppresses aggregation of proteins and so inhibits protein precipitation.