20 October 2014 4 2K Report

We want to determine the concentration of cGMP in ventricular tissue, in order to evaluate the activity of the Soluble Guanylate Cyclase enzyme.

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Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

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Cheaper alternative to XSens mocap system for ergonomic data acquisition?

Hello all, I'm starting a project where we want to automate the video analysis of people working in different environments to produce ergonomic measures like hip flexion, shoulder extension, etc....

16 June 2024 1,843 1 View

Does anyone have experience with immunofluorescence of endoplasmatic reticulum?

Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...

06 June 2024 7,764 1 View

How can I change the coordinate system in Abaqus using Python?

Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...

30 May 2024 7,431 3 View

How can I induce neuroinflammation with LPS in HMC3 cells ?

dear all, I try to induce neuroinflammation in HMC3 cells using LPS for 24h. The concentration used was 500 ng/ml. But unfortunately no change on TNFa was obtained in LPS versus Control...

29 May 2024 4,007 1 View

Are the grey/white bands composed of magnetite and haematite or are there more iron oxdies/suplfides?

This is a Fe-concretion (about 2 cm in diameter) of possible sedimentary origin (Carpathian Flysch; reflected light microscopy). I suppose the dark grains are could be quartz, subsequently...

22 May 2024 7,954 2 View

Does anyone know of a validated questionnaire on self-efficacy in health professionals / medical doctors?

I am searching for a validated measurement instrument to assess self-efficacy in health care professionals / medical doctors / general practitioners regarding their tasks in medical care and...

21 May 2024 656 0 View

Biobase BKQ-B120L autoclave issues?

I am trying to get a new Biobase BKQ-B120L autoclave to work however it does not go through its cycles. It will start up and go to about 110C and then remains there untill the ERR alarm sounds....

21 May 2024 4,886 1 View

Why do I get frameshift mutants in antibody phage display?

I'm performing an antibody phage display with a VHH library and I consistently get frameshift mutants (mainly frame +2) after biopanning. I'm using TG-1 cells for amplification of phagemids and...

14 May 2024 412 2 View

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