Glucose enter into the RBCs and get converted into glucose-6-phosphate, this glucose-6-phosphate is responsible for non-enzymatic glycosylation.... which leads to the further diabetic complications. In-vitro studies show that glucose-6-phosphate is producing more glycosylation than the glucose.
What will happen if we add glucose-6-phosphate into the isolated and maintained human RBCs?
Thank You Salvatore Sir..... This information will help me to clear some doubts.... I want to plan some in-vitro experiments using RBCs, for this should i use only glucose or glucose and glucose-6-phosphate together in order to mimic the diabetic condition?... i have found the reference stating that elevated levels of the glucose-6-phosphate are found in diabetes patients RBCs... bt i am not clear about, whether the glucose- 6- phosphate is elevated only in RBCs or RBCs are exposed to free glu-6-phosphate in diabetic patients? .... that may lead to direct entry of glu-6-phosphate into the RBCs..... bt i m not sure......
Respected Salvatore Sir
Thank you for your detailed explanation for the increased glucose-6-phosphate levels in diabetic erythrocytes. This clears my most of the queries. I have gone through most of the papers referred by you. Your reply has given me a strong background for our study.
Thank You again for your kind guidance. I will be in touch with you as our work proceed.
One reason for phosphorylation of carboydrates like glucose is to keep them in the cell. A study that I did many years ago (unpublished) clearly demonstrated this as I was trying to use serum G6P as a marker of tissue glycolysis. It didn't work! It is unlikely G6P will cross into erythrocytes, unless there is a specific transporter on the plasma membrane?
Thank You Craig Atwood Sir for sharing your experience with us......
It will prevent us from jumping into any untoward conclusion.
Thank You Savatore Sir. This is really a very interesting information. I will go through it.
Both the reference added by you are very useful and informative. Thanks for your valuable guidance.
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