Glucose enter into the RBCs and get converted into glucose-6-phosphate, this glucose-6-phosphate is responsible for non-enzymatic glycosylation.... which leads to the further diabetic complications. In-vitro studies show that glucose-6-phosphate is producing more glycosylation than the glucose.
What will happen if we add glucose-6-phosphate into the isolated and maintained human RBCs?
Thank You Salvatore Sir..... This information will help me to clear some doubts.... I want to plan some in-vitro experiments using RBCs, for this should i use only glucose or glucose and glucose-6-phosphate together in order to mimic the diabetic condition?... i have found the reference stating that elevated levels of the glucose-6-phosphate are found in diabetes patients RBCs... bt i am not clear about, whether the glucose- 6- phosphate is elevated only in RBCs or RBCs are exposed to free glu-6-phosphate in diabetic patients? .... that may lead to direct entry of glu-6-phosphate into the RBCs..... bt i m not sure......
Respected Salvatore Sir
Thank you for your detailed explanation for the increased glucose-6-phosphate levels in diabetic erythrocytes. This clears my most of the queries. I have gone through most of the papers referred by you. Your reply has given me a strong background for our study.
Thank You again for your kind guidance. I will be in touch with you as our work proceed.
One reason for phosphorylation of carboydrates like glucose is to keep them in the cell. A study that I did many years ago (unpublished) clearly demonstrated this as I was trying to use serum G6P as a marker of tissue glycolysis. It didn't work! It is unlikely G6P will cross into erythrocytes, unless there is a specific transporter on the plasma membrane?
Thank You Craig Atwood Sir for sharing your experience with us......
It will prevent us from jumping into any untoward conclusion.
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