In an order to check for DNA protection on X-ray/gamma irradiated DNA samples treated with any radioprotective drug, plasmid relaxation assay is often been cited as an easy tool, where plasmids of known sizes like pBR322 are commonly used. Although the assay is specific for plasmids as it is based upon the percentage of appearance of the supercoiled and open circular forms which is not possible in genomic DNA. Is there a possible way to do the same assay with genomic DNA of any other origin for checking DNA protection with radioprotective drugs on the basis of appearance of bands on agarose gel.
The experiment is strictly based on naive DNA only without any cell system so the question of COMET assay etc., is totally ruled out for me.
Our article published in peer-reviewed Journal "Communicative & Integrative Biology". A few major points discussed in the paper:
(1) Brain is not the source of consciousness.
(2) Consciousness is ubiquitous in all living organisms, starting from bacteria to human beings.
(3) The individual cells in the multicellular organisms are also individually cognitive entities.
(4) Proposals like “artificial life”, “artificial intelligence”, “sentient machines” and so on are only fairytales because no designer can produce an artifact with the properties like internal teleology (Naturzweck) and formative force (bildende Kraft).
(5) The material origin of life and objective evolution are only misconceptions that biologists must overcome.
http://www.tandfonline.com/doi/full/10.1080/19420889.2015.1085138
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology