Can dendritic cells uptake, and process live tumor more cells?

01 January 2018 0 7K Report

Hello everyone,

I am wondering, what will be happened if culture dendritic cells (DC) with live cancer cell line? Can DC uptake and process these live cancer cell lines to be peptides?

Thank you!

Badges
Science topic

More Cuong Manh Vo's questions See All

Does anti-PD-L1 antibody kill dendritic cells?

Dear all, Please help me to solve this question, Because, PD-L-1 is well-know high expressed on cancer cells and antigen presenting cells, such as dendritic cells (DCs), using PD-L1 antibody can...

07 August 2017 1,846 2 View

What kind of Non-small cell lung cancer (NSCLC) cell lines are sensitive to NK cells?

Hi everyone! I am preparing to do the efficacy of human NK cells in Non-small cell lung cancer (NSCLC) Scid mouse model, and now I am finding which kind of NSCLC cell lines are sensitive to NK...

06 July 2016 7,707 2 View

Does TLR5 express on or in MC38 cell lines?

Dear everybody, Plz help me, Now I am doing in vivo mouse tumor model by using dendritic cells combined with v-FlaB (as adjuvant) against colon cancer model (MC-38 cells). but some papers mention,...

08 September 2015 5,246 5 View

How do you explain IL-12p70 production from immature dendritic cells being higher that mature dendritic cells?

Hi everyone, can you help me to answer this question. I am culturing human dendritic cells (DCs) from blood monocytes, And I cocultured immature DCs and mature DCs with J558-CD40 Ligand cell...

02 March 2015 2,398 6 View

Cell homing and cell migration?

in immunology, what is different between cells migration and cells homing? Thank so much!

01 February 2015 3,598 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View