Can defrosted muscle sample be used to valuate myofibrillar fragmentation index?

03 May 2019 0 5K Report

I have the necessity to evaluate myofibrillar fragmentation index to study meat's maturation. Can I performe the method on thawed meat sample?

Badges
Science method

More Rina Verdiglione's questions See All

How to assess significance of gene mutation burden in Endometrial Cancer data collated from different studies?

I am trying to analyse mutation data for endometrial cancer obtained from different studies within several databases (COSMIC, cBioportal, Intogen). I have collated the data and grouped the...

29 August 2023 4,992 4 View

DSAS : unable find output file DSAS "C:\Users\ ...\AppData...." ?

when calculate the rates the attribute table of the transect_rates it doesnt work. It desnt appear the calculate of nsm, epr, lrr. Does anyone know what the solution is? Thank you

03 June 2023 3,886 1 View

Has anyone had problems with Modular Cloning when cloning multiple big inserts?

Hi everyone, Has anyone had problems with Golden Gate when cloning multiple big inserts? My backbone’s size is 7254 bp and each of my insert is approx. 5000-5700 bp (I have 4 inserts) and the size...

11 May 2023 6,002 1 View

Definition of fluorescent intensity mean ?

Dear researchers, I'd like to quantify my fluorescent images. I want to compare 2 different regions, one is bigger in size and the other smaller. and I measure the fluorescent intensity mean in...

22 March 2023 7,713 1 View

Greeting everyone, I need to conduct a mediation analysis on panel data. Is everyone aware mediation analysis suitable to conduct?

My data involves 3 different time points with 2 dependent var, 2 independent var, 1 mediator, and 1 control variable. Each of the variables is latent except for the control variable. I will use...

08 February 2023 459 1 View

Deas anybody know, what statistical software can beu use for Longitudinal Structural Equation Modeling?

I am working on panel data measuring the direct and indirect effects of social support on subjective well-being from a retrospective cross-sectional data

02 February 2023 3,419 4 View

How can I solve undertrypsinzed in the slide? Can anyone give me some ideas to get good band in G-banding?

I already follow some troubleshoot in AGT book and some of cytogenetic's journal. I have tried to change the concentration of trypsin and giemsa but I still dont get the band (see the...

20 December 2018 5,486 9 View

How to prepare p-anisidine reagent for fatty acids?

Fatty acid, method

27 February 2018 1,841 4 View

Which type of motivation (Intrinsic/Extrinsic) is more dominant at learners of ESL?

ESL- English as a Second Language

15 December 2017 3,027 9 View

What is the difference between repeated cross sectional study and pseudo longitudinal study?

26 February 2017 3,413 12 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

Does EMG show muscle spasms?

Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?

20 July 2024 5,951 1 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View

How can I increase DNA concentration after gel purification for long length DNA fragment?

I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...

01 July 2024 4,912 9 View

Please, what are the recent/top/free software for predicting data from MALDI-TOF that you have tried?

Please, can the fragmentation obtained from the mass spectrometry be predicted more easily using any free software? Kindly recommend it, thanks.

01 July 2024 5,327 6 View