How can I solve undertrypsinzed in the slide? Can anyone give me some ideas to get good band in G-banding?

I already follow some troubleshoot in AGT book and some of cytogenetic's journal. I have tried to change the concentration of trypsin and giemsa but I still dont get the band (see the...

20 December 2018 5,384 9 View

Which type of motivation (Intrinsic/Extrinsic) is more dominant at learners of ESL?

ESL- English as a Second Language

15 December 2017 2,828 9 View

Is pretreatment important for food processor? Why?

17 December 2016 1,555 18 View

What are the chemical preservative used in mayonnaise?

11 December 2016 362 15 View

What kind enzyme be able to degrade PHAs film as biodegradable polymer?

Could I use lysozyme or lipase? hopefully I can buy from sigma or other chemical company. Thank you

03 October 2016 739 2 View

What is the safety amount of benzoyl peroxide allows in food, microbiology and chemically?

04 July 2016 7,633 3 View

Is there a software to calculate substituent constant?

Dear all, I was trying to calculate the Hammett constant of some special groups (no experiment data). while the reviewer told me it could be done by software. what he/she said was 'approach...

17 December 2015 4,803 3 View

What do we call the molecular skeleton?

For example, a substituted benzoic acid is composed of three parts,the functional group,the substituent, and the benzene ring.what do we call the benzene ring, or the general structure that has...

18 November 2015 6,578 3 View

I have a question about the thermal melting of DNA organic molecule hybrid structures?

04 September 2014 6,178 3 View

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11 August 2014 2,464 3 View

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02 March 2021 3,060 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How to incorporate Monte Carlo simulation in for a real trading data?

Hello, My area of research is to valuate Exchange option (Margrabe option). I am looking for Monte Carlo simulation technique that can be applied to some real trading data. I have evaluated them...

05 February 2021 1,605 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View