Dear researchers,
I'd like to quantify my fluorescent images. I want to compare 2 different regions, one is bigger in size and the other smaller. and I measure the fluorescent intensity mean in these two regions. Should I divide the fluorescent intensity mean with area of region?
I would suppose that the fluorescence intensity mean for a region is the average pixel intensity for the region, in other words, the sum of the intensities for all the pixels divided by the number of pixels. Therefore, you do not need to divide by the area of the region because the area of the region is the same as the number of pixels in the region, so the division has already occurred. If you want to compare the total intensities of two regions, you would have to multiply their average intensities by the number of pixels or area of the regions.
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