Can Colony PCR be applied to genomic DNA, or only for ligation (vector/insert) screening?

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Dr. Godfred A. Menezes

You can apply colony PCR for genomic DNA too.

Shajia Afrin

Hi Miriam, you can do colony PCR for genomic DNA.

Giorgio Aglieri

Hi Miriam, you can definitely do a colony PCR for genomic DNA. It is a easy way to proceed.

Irfan Arif

Sure you can. Only thing you might wanna change is the unusual long heating step in the start of a colony PCR which you can reduce in case of genomic DNA.

Nathaniel L Hepowit

Colony PCR is also applicable for genomic DNA. This is one of the ways I usually do in screening null mutants and DNA-region integrants.

Hossein Khodadadi

Hi Miriam

You could find some useful tips in article:

Colony-PCR Is a Rapid and Sensitive Method for DNA Amplification in Yeasts

H Mirhendi et al.