Miriam Khider

7 Questions 15 Answers 0 Followers

Questions related from Miriam Khider

How to use RNALater with bacterial cultures?

Dear, I am working with a slow growing bacteria. I need to harvest 10 ml at the low OD and approximately 5ml at high OD for RNA isolation and transcriptomics. I saw that most people use RNA...

29 March 2017 578 7 View

Fast Cloning, Help to understand the method of primes?

I am doing this for the first time and I need a quick check wether i have understood the method correctly. My plasmid has a CAM gene which is going to be deleted and replaced with KAN. 1. Primer...

22 February 2017 810 6 View

Help with electroporation?

I am following some electroporation protocols found in the literature. Using Genepulser X cell from BioRad and cuvettes 0.2 cm (2mm) The V (voltage) in the instrument is from 160V to 2500V How to...

24 July 2014 1,366 4 View

Can Colony PCR be applied to genomic DNA, or only for ligation (vector/insert) screening?

25 June 2014 7,413 6 View

Can anyone help with transcriptional and translational fusion?

For transcriptional fusion do you clone the promoter of interest upstream of the reporter gene which is promoterless to study the promoter strength? And in translational fusion do you clone the...

30 April 2014 8,048 5 View

How to calculate the concentration of PCR product from DNA mass standard on gel (e.g.1kb from neb) for sequencing reaction?

I need to use 1 ul of 3-10 ng of my pcr product to sequence by BigDye 3.1 protocol. How can i measure the concentration of my pcr product out of the gel ladder/standard?

11 November 2013 853 8 View

Overlapping PCR?

I need help with overlapping PCR, I have 4 primers A, B, C and D where B and C have complementary bases. 1. first PCR run with Phusion Polymerase from Thermo F-530S to generate PCR product AB and...

07 November 2013 765 14 View