Can Co-IP be performed by using lysates from tissues that over express your protein of interest?

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Indrayani Waghmare

Thanks :) I appreciate your help.

Praveen Jawanmal Khandelwal

Yes you can use lysate form tissue for CO-IP, but i would say before performing better check the present of protein in your lysate by western blotting further proceed to coip. COIP experience with me was, protein present in IP but not in COIP so i have to increase the protein concentration in COIP to get the suitable band.

Thank you