NF Kb estimation: any available biomolecule in serum which can estimate the expression of NF-KB?

nuclear factor kappa light chain enhancer of activated B cells which gives estimate of cytokine release, ultimately inflammation . Is there any diagnostic kit is available which can estimate its...

28 July 2019 1,864 5 View

Which parameter does control stiffness degradation in ABAQUS Concrete Damaged Plasticity Model for triaxial Loading case?

I am trying to verify the experimental results on triaxial compressive loading on concrete cylinder using Concrete Damaged Plasticity Model, but I am not able to get results as expected.. Getting...

16 September 2018 2,765 5 View

Is pAc5.1 plasmid low copy number plasmid?

I consistently get low yields with pAc5.1 constructs. I have tried growing cultures from fresh plates and glycerol stocks. I have also tried to use starter cultures (grown for 8 h) and I always...

05 June 2018 8,204 3 View

Is there any mechanism in living organisms to code a new DNA sequence for new substance which is not present any other organism.

If a organism found a compound (protein, lipid or polysaccharide) which is very useful for it. But sequence for synthesizing that product is not present in that organism. Is there any natural...

03 June 2017 858 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View