Indrayani Waghmare

4 Questions 3 Answers 0 Followers

Questions related from Indrayani Waghmare

Is pAc5.1 plasmid low copy number plasmid?

I consistently get low yields with pAc5.1 constructs. I have tried growing cultures from fresh plates and glycerol stocks. I have also tried to use starter cultures (grown for 8 h) and I always...

06 June 2018 8,355 3 View

What's the protocol for performing Heparinase III treatment on S2 cell lysates?

I used Hep III (from Sigma, reconstituted using manufacturer's protocol) and incubated the lysates with Hep III (final concentration 2U/ml, 4h at 37). But this gives me precipitate at the end of...

01 December 2016 6,275 3 View

How might one get clean western blots?

Hi, I have been trying to probe this blot with 6 antibodies, anti p-JNK, yap, p-Yap, Caspase9, Wg and tubulin. The first 4 are generated in Rabbit (cell signaling). Other than the obvious issue of...

03 November 2014 8,253 7 View

Can Co-IP be performed by using lysates from tissues that over express your protein of interest?

I have seen most people use cell-cultures that over-express a specific construct. Thanks.

12 October 2014 5,672 2 View