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I consistently get low yields with pAc5.1 constructs. I have tried growing cultures from fresh plates and glycerol stocks. I have also tried to use starter cultures (grown for 8 h) and I always...
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I used Hep III (from Sigma, reconstituted using manufacturer's protocol) and incubated the lysates with Hep III (final concentration 2U/ml, 4h at 37). But this gives me precipitate at the end of...
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I have seen most people use cell-cultures that over-express a specific construct. Thanks.
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