nuclear factor kappa light chain enhancer of activated B cells which gives estimate of cytokine release, ultimately inflammation . Is there any diagnostic kit is available which can estimate its level in serum sample.
I do not believe there is a diagnostic assay for NFkB, but there are certainly research use only (RUO) assays. However, I would be hesitant when considering a NFkB RUO ELISA; NFkB is a intracellular family of transcription factors (https://www.creative-diagnostics.com/The-NF-kB-Signaling-Pathway.htm).
Each family member can form heterodimers, with each dimer set having different regulatory functions. Thus the question should be which heterodimer is of interest, rather than NFkB. Further, since the NFkB is intracellular (cytoplasmic and nucleolar), serum would not be a good matrix to assess NFkB heterodimers in. Typically you would want to use cell lysates.
Overall, there seems to be no diagnostic assay for serum NFkB, with RUO assays targeting a specific heterodimer. Further, serum is not the matrix of choice for NFkB analysis. I was unable to find much really on soluble circulating NFkB.
There are plenty NFkB elisa kits commercially avilable for different organisms. Simply google.
I do not believe there is a diagnostic assay for NFkB, but there are certainly research use only (RUO) assays. However, I would be hesitant when considering a NFkB RUO ELISA; NFkB is a intracellular family of transcription factors (https://www.creative-diagnostics.com/The-NF-kB-Signaling-Pathway.htm).
Each family member can form heterodimers, with each dimer set having different regulatory functions. Thus the question should be which heterodimer is of interest, rather than NFkB. Further, since the NFkB is intracellular (cytoplasmic and nucleolar), serum would not be a good matrix to assess NFkB heterodimers in. Typically you would want to use cell lysates.
Overall, there seems to be no diagnostic assay for serum NFkB, with RUO assays targeting a specific heterodimer. Further, serum is not the matrix of choice for NFkB analysis. I was unable to find much really on soluble circulating NFkB.
Gion Fränkl is right. Most commercially available kits (almost all of them research grade) measure total NFkB and that too in cell lysates. Some mention serum and plasma as applicable sample types but I highly doubt how meaningful the data would be even if you manage to detect it in serum. Being a transcription factor, the more relevant aspects to look at would be its sub-cellular location and phosphorylation status. A serum NFkB ELISA would not give info on either of these. You could try measuring surrogate markers like cytokines or other soluble molecules in the serum but ensuring you select one that is specifically induced by NFkB (and not by other signaling pathways) is going to be tough.
The available Elisa kits are more than good enough to estimate serum levels. In most cases RUO is merely A cost decision in function of clinical validation costs. So if not for real diagnostic purposes I see no problem. Obviously your other remarks are definitely of interest.
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biomolecules