What is the reason that the binding energy of a docked complex is negative?

I have two questions: The binding energy of complex is negative. Why? can u suggest any tools to determine the interacting energies for a docked complex?

06 July 2018 9,987 0 View

What is the best absorbance value of Agrobacterium culture to be used to infect plant callus?

I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...

01 February 2018 863 1 View

I carried out patchdoc for Protein-DNA and got the result but i need a tool for find interaction energy

06 July 2017 4,604 2 View

Why do I get contaminations after two-three weeks sucessful plant callus growth?

I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...

31 December 2015 5,403 11 View

Why is E.coli BL21(DE3) strain used in eukaryotic expression?

08 September 2015 4,297 4 View

How can I use the software "Map Inspect" to find locations of genes?

I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...

04 May 2015 9,668 1 View

How can one estimate Salicylic acid and Jasmonic acid in plants using calorimetry?

How to determine calorimetrically? HPLC methods exist but can one determine using UV spec or fluorometry?

09 October 2014 1,901 3 View

What is the mechanism of WRKY transcription factor against biotic stress?

What is the probable mechanism of WRKY Transcription factor or the pathway in which wrky plays a major role against biotic stress. Please provide references.

08 September 2014 6,141 7 View

What are the new advanced techniques in plant molecular biology involved in proving disease resistance?

Need to know new methods which can help in proving the role of a particular gene in a specific function of the gene.

05 June 2014 3,523 2 View

Can fixing the gel be a problem for no spots in your 2D gel?

I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.

04 May 2014 2,693 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View