Good morning, I plan to introduce the expression of a human glutamate dehydrogenase (GDH) mutant (the most frequent mutation associated with epilepsy and hyperinsulinism in human). For that an adenovirus construct for the delivery of transgene was produced before my arrival in the lab. The problem I have is that the GDH mutant don’t have any flag or is not coupled to GFP, the vector is not bicistronic too. Consequently if I injecte the adenovirus by stereotaxy I will not see the injection site. Do you think I can co-infuse an adenovirus mito-RFP to identify where I injecte the adenovirus GDH? Do you know if astrocytes and neurons can be simultaneously infected by 2 adenovirus in vitro and in vivo, or if the cells develop defensive mechanisms?
Dear Chloé Chrétien
Pls. find the attached files
http://www.sciencedirect.com/science/article/pii/S0092867400807837
https://www.ncbi.nlm.nih.gov/pubmed/8635562
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701857/
https://www.researchgate.net/post/Adenovirus_transduction_in_glia_vs_neurons
http://www.nature.com/gt/journal/v8/n17/full/3301529a.html
page 595 in: Bernardo Castellano, B. Castellano López, Manuel Nieto-Sampedro. 2003. Glial Cell Function. Gulf Professional Publishing,Pp.757. ISBN0444514864, 9780444514868
http://abbs.oxfordjournals.org/content/41/4/325.full
http://www.nature.com/cr/journal/v22/n2/full/cr2011185a.html
http://search.proquest.com/openview/e500b0bf44544f62d86e3c80cab4e482/1.pdf?pq-origsite=gscholar&cbl=1256
Hoping this will be helpful
Regards
Prof. Houda Kawas
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