Can anyone tell me how to perform protoplast fusion of yeast cells?

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Vivek Kumar Morya

A number of methods know for yeast protoplast fusion, now a days several kits are also available for such experiments. the following links may help you.

http://www.aidic.it/IBIC2008/webpapers/96Verma.pdf

http://www.amsbio.com/protocols/Zymolyase_Protocols.pdf

http://eshop.eppendorf.ca/documents/download/Protocol_Yeast_Protoplasts_Protocol_No_4308_91_045131_Protocol.

http://www.amsbio.com/zymolyase.aspx

NILESH KUMAR SHARMA

Dear Dr. Vivek Morya,

Thank you very much for your support.