I want to do Indirect ELISA to measure antibody titer.
I would coat my protein to the ELISA plate, which is more efficient between carbonate buffer and PBS?
What's the difference in function?
I know the carbonate buffer's PH is 9.6, so does it effect on my protein?
Mostly the coating woks in both buffers. Why pH 9.6? Simply most of the amino acid residues are neutralized and this will help the protein to interact via hydrophobic interaction with the polystyrole surface of the plate. This reaction need some time (to get the water away from the protein and the plate. Therefore overnight and at 4 °C
Dear Stephan,
What is exactly the solvent in the bicarbonate buffer? And is the plastic surface of the plate hydrophobic or hydrophilic?
Thank you in advance,
Par
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