YINGJI JIN

8 Questions 10 Answers 0 Followers

Questions related from YINGJI JIN

How to use bonferroni correction?

I don't know how to adjust bonferroni correction to my data. Firstly, I tested the levels of several types of biomarker candidates in normal and cancer serum using ELISA. I found three types of...

08 August 2017 3,883 4 View

Can I check the specificity between glycan and Lectin as below design?

Firsty, I found that fucosylation level of anti-A protein antibody is significantly lower than anti-B protein antibody with ELISA using lectin. (Coating: A or B protein, Blocking, Sera,...

12 December 2015 3,742 1 View

How can I count Liquid based cytology cell?

Hello I have a really confusing question. I wanna count the cell of liquid based cytology(LBC). But the cell morphology is different between normal LBC and cancer LBC. And normally, cancer LBC...

02 February 2015 976 5 View

Can I use unstained cells as negative controls for FACS analysis?

Now, I wanna compare the expression level of cell surface protein between normal cell and cancer cell. I would add FITC conjugated antibody to detect this protein, but I have no idea about the...

01 January 2015 6,822 17 View

How do I couple proteins to cnbr-sepharose for making a protein specific resin?

Firstly, I wanna purify one kind of antibody from serum which is specific to one kind of protein. Ex) protein XX. So, I coupled this protein XX to CNBr sepharose resin to make protein XX affinity...

01 January 2015 9,949 8 View

Can anyone tell me about the difference between carbonate buffer and PBS coating buffer at Indirect ELISA?

I want to do Indirect ELISA to measure antibody titer. I would coat my protein to the ELISA plate, which is more efficient between carbonate buffer and PBS? What's the difference in function? I...

09 September 2013 5,776 4 View

Can anyone tell me about the difference between ELISA antibody and Western blotting antibody?

Some antibodies are just used for western blotting, and some antibodies are just used for ELISA. So what is the difference? I producted serum antibody with Virus particle which has several major...

09 September 2013 4,291 7 View

How can I use SDS-PAGE gel to immunize a mouse?

I am trying to immunize a mouse with proteins but the proteins are proving hard to purify. I want use SDS-PAGE gel to seperate the proteins and immunize the protein with gel. How can I do this?...

08 August 2013 9,692 13 View