Does anyone get full length ABCA1 protein band in Raw264.7 cell lysate?

I have been trying westren blot against anti-ABCA1 (tried Novus Biological and abcam antibodies both.). LXR activated cells do show 220kda ABCA1 band. But control cells show 120 KDa band exact...

03 April 2015 7,546 3 View

Does anyone have experience with HepG2 cells?

Something is happening in my lab. Cells were not split for a long time and then saw all flask are turbid and then some black tiny structures are overcrowded in all the flask. Next day, changed...

04 May 2014 7,699 4 View

What should the binding buffer composition be for binding purified protein with DNA?

I want to check whether or not my protein binds to DNA. At this stage, I am not using any labeling. I want to see if there is any DNA-protein interaction. Please suggest the ideal binding buffer...

03 April 2014 5,250 4 View

Would could cause the viability of HepG2 cells remain 50% after plating cells?

HepG2 cells viability checked by Trypan blue dye was 855 before plating cells. After 24 hours of adhesion, I incubated cells for another 6 hours, 12 and 24 hours. Viability of cells reduced to 50%...

02 March 2014 5,014 9 View

Is it necessary to pretreat cells with serum free media for 12-24 hours before treatment with inducer?

I am treating HepG2 cells with Lipoprotein. I seed cells in serum media (10%). Just before treatment, I change media to serum free media. Should I maintain cells in serum free media for 12 hours...

01 February 2014 8,174 5 View

Can anyone help with hepG2 cells SiRNA transfection?

I am using HepG2 cells for transfecting SiRNA. I have got SiRNA from sigma along with nanoparticle based transfection reagent. I am getting almost negligible knockdown of my gene of interest...

10 November 2013 3,228 9 View

Any suggestions for treating DMSO soluble compound in cell culture?

I am using DMSO soluble compound to treat HepG2 cells. The problem is when I dilute DMSO soluble compound in water to get the desired concentration for treating cells, I am getting precipitation...

10 November 2013 3,484 22 View

Can I block a receptor with specific antibody in HepG2 cells?

I am going to block receptor with specific antibody at 37 degrees for 24 hours and then going to treat my cells with stimulant. Will that work for blocking receptor?

07 August 2013 7,981 12 View

Can I treat MG132 and stimulant together on macrophage cells?

I have read that MG132 should be pretreated , while few treated together. Which option will be better?

06 July 2013 2,481 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View