Monika Sharma

10 Questions 34 Answers 0 Followers

Questions related from Monika Sharma

Does anyone get full length ABCA1 protein band in Raw264.7 cell lysate?

I have been trying westren blot against anti-ABCA1 (tried Novus Biological and abcam antibodies both.). LXR activated cells do show 220kda ABCA1 band. But control cells show 120 KDa band exact...

04 April 2015 7,625 3 View

Does anyone have experience with HepG2 cells?

Something is happening in my lab. Cells were not split for a long time and then saw all flask are turbid and then some black tiny structures are overcrowded in all the flask. Next day, changed...

05 May 2014 7,756 4 View

What should the binding buffer composition be for binding purified protein with DNA?

I want to check whether or not my protein binds to DNA. At this stage, I am not using any labeling. I want to see if there is any DNA-protein interaction. Please suggest the ideal binding buffer...

04 April 2014 5,312 4 View

Would could cause the viability of HepG2 cells remain 50% after plating cells?

HepG2 cells viability checked by Trypan blue dye was 855 before plating cells. After 24 hours of adhesion, I incubated cells for another 6 hours, 12 and 24 hours. Viability of cells reduced to 50%...

03 March 2014 5,077 9 View

Is it necessary to pretreat cells with serum free media for 12-24 hours before treatment with inducer?

I am treating HepG2 cells with Lipoprotein. I seed cells in serum media (10%). Just before treatment, I change media to serum free media. Should I maintain cells in serum free media for 12 hours...

02 February 2014 8,260 5 View

Any suggestions for treating DMSO soluble compound in cell culture?

I am using DMSO soluble compound to treat HepG2 cells. The problem is when I dilute DMSO soluble compound in water to get the desired concentration for treating cells, I am getting precipitation...

11 November 2013 3,556 22 View

Can anyone help with hepG2 cells SiRNA transfection?

I am using HepG2 cells for transfecting SiRNA. I have got SiRNA from sigma along with nanoparticle based transfection reagent. I am getting almost negligible knockdown of my gene of interest...

11 November 2013 3,293 9 View

Can anyone suggest why I am having trouble with my HepG2 cells?

HepG2 cells are differentiating just like macrophage cells in quite early passage, Passage 5.

09 September 2013 8,450 3 View

Can I block a receptor with specific antibody in HepG2 cells?

I am going to block receptor with specific antibody at 37 degrees for 24 hours and then going to treat my cells with stimulant. Will that work for blocking receptor?

08 August 2013 8,042 12 View

Can I treat MG132 and stimulant together on macrophage cells?

I have read that MG132 should be pretreated , while few treated together. Which option will be better?

07 July 2013 2,580 9 View