Does anyone get full length ABCA1 protein band in Raw264.7 cell lysate?

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Huan Hu

Hi Monika~

in Abcam website they said: Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, it means that 120 kDa position may the non-specific band,

Monika Sharma

Yes, I reckon so. But the question is why there is no full length band which people do show in their papers?

well , Monika,

first i recommend you do a ponceau S staining to identify your bands position, and then just do a full control sample to confirm that whether all the control cells show 120 KDa band...

Hope this help~