RAD sequencing
A D'Hont
Heslop
GBS works well for allopolyloidy species without reference genome. We use GBS to discover genome-wide SNP markers and then perform genomic selection.
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Greetings, I am a PhD Scholar, I want to analyze the metabolites present in different tissues of bitter melon plant (Momordica charantia). I have grown the plants and I have the plant sample ready...
28 August 2023 764 2 View
In many articles, the exchange current (i₀) value for the volcano plot can be found from the Norskov's assumption. When I used their values, I couldn't achieve their results. The exchange current...
08 August 2023 6,641 0 View
Dear experts, We have analyzed the FOXP3 gene mutation of 10 healthy volunteers and 13 diseased samples. Out of these, 3 healthy volunteers (30%) and 8 diseased patients (61.53%) were found to...
21 July 2023 1,365 2 View
I want to synthesize high molecular-weight polyacrylamide. I tried synthesising PAM using acrylamide, 2-acrylamido-2-methylpropane sulfonic acid in alkaline median like ph 7-8. In this case, I...
13 June 2023 2,086 4 View
Dear Expert, We have a single group of data consisting of 520 volunteers. All of them were smokers and protected from certain diseases. In such a scenario, we would appreciate your guidance on how...
04 June 2023 8,802 5 View
Dear experts, Our team is working on a new intervention for drug dependence and we require information on the volume of distribution (Vd) for ascorbic acid in humans for our project. Would you...
08 April 2023 1,259 0 View
I need to collect mosquito larvae from the environment.
03 March 2023 2,966 4 View
Dear Kinetic experts, I need one kind suggestion from you. One X compound with molecular weight of 200.1, is highly water soluble with oral bio-availability of 65-75%. In that case I want to...
02 February 2023 9,696 4 View
Since acetic anhydride is not available in our country, What can be the alternative of Acetic anhydride for acetylation of carbohydrates
13 December 2022 3,047 0 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Please go through my Abstract. I can also share a proposed Thesis Outline.
04 August 2024 2,077 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View