Since acetic anhydride is not available in our country, What can be the alternative of Acetic anhydride for acetylation of carbohydrates
I am trying to express HIV1 antisense protein in E.Coli. The protein is hydrophobic and also has two N-terminal cysteine triplets. I am planning to add a pelb signal peptide for periplasmic...
31 January 2024 1,862 1 View
I am using cobalt column for purification. If anyone has done it, kindly share the protocol.
24 January 2024 2,928 0 View
Plasmid Concentration - 25ug per t75 flask Cell number - 10 million cells Protein size - 25 KDa Purification procedure - His tag purification using cobalt resin. I am going to use this purified...
15 November 2023 7,015 1 View
I need to collect mosquito larvae from the environment.
03 March 2023 2,966 4 View
I have modelled a Beam and Car using Abaqus for impact analysis. During the analysis, the Car passes through (Penetrates) the beam without any impact. Can anyone please give me any valuable...
02 April 2022 6,676 2 View
I was constructing marker free vector for Pichia pastoris , but I got a problem when adding CRE gene under AOX promotor which is showing leaky expression. Please help me with ur valuable suggestions ?
02 August 2021 3,014 2 View
Considering that Covid has manifested in various forms and it is expected that this wave will end only by the starting of 2022. In this context, there is likelihood of HR force working mostly from...
12 May 2021 2,224 5 View
Dear researchers, To calculate bandgap using Tauc relation from UV Abs data of thin-film samples, firstly we need to have Absorption coefficient which is calculated from absorption coefficient...
28 April 2021 2,889 5 View
I am trying to train a UNET architecture built with the PyTorch library. I am looking for the best methods to load my data into the model. the dataset has around 500 images (RGB image of around...
18 March 2021 8,602 2 View
Adsorbents are used to remove pollutants from water. How the removed pollutants disposed off? If the pollutants are dumped, how adsorption is considered as sustainable.
01 December 2020 5,408 11 View
If they can be acetylated and methylated what will be the effect?
02 July 2024 4,030 2 View
I prepared h-BN by hydrothermal methods by boron anhydride and urea after hydrothermal I was heated at 800 temps for 12 hours after that my sample stuck to the crucible the third time, and the...
15 May 2024 7,354 0 View
Hi all! I would like to know if there is a method to polymerize low MW cellulose triacetate (e.g. re-engaging in acetylation). Or this is not possible and the MW of my cellulose source should be...
08 May 2024 5,260 1 View
Basically, I have deprotected a BOC, TPDMS-Protected sphingolipids using Acetyl Chloride, which gave a salt of deprotected sphingolipids. Can salts run on TLC?!
25 April 2024 2,971 1 View
Deacetylation of chitin results in different de acetyl chitin (chitosan)with various degrees of de acetylation.Such variation produces a mixture of different( chitosans) which is not studied...
02 April 2024 2,739 0 View
what is 1335 in acetyl value and why this value put here as constant? what is 1335 in acetyl value and why this value put here as constant?
10 January 2024 8,357 0 View
Does Acetyl Chloride (AcCl) is best alternative for acetic anhydride for acetylation reaction?
04 January 2024 3,573 1 View
The imidation of nitronaphthalic anhydride with an amino compound is conducted using glacial acetic acid. Will this procedure yield the desired product?
31 December 2023 9,429 1 View
What are some important nmr peaks where tetra and penta saccharides give characteristics nmr signals. I have acetylated oligosaccharide. Please suggest only nmr elucidation of 1H and C13
15 November 2023 3,677 0 View
I am working on methacylation of polysaccharides and came across a research paper that used a certain amount of methacrylic anhydride was added based on a calculation that used 5 Molar equivalent...
13 August 2023 8,052 0 View