Since acetic anhydride is not available in our country, What can be the alternative of Acetic anhydride for acetylation of carbohydrates
Can anyone help me to identify miRNAs from Assembled Transcriptome (multi fasta contig files around 45,000). I checked with mirBase but it has some limitations. I did a local BLAST using mature...
28 January 2020 4,454 3 View
Im using wild type SW620 cancer cell line and shCD25 SW620 cells. I want to show the significant difference in the cell viability between both the cell lines to the chemotherapeutic drug...
24 August 2019 9,807 5 View
I want to analyse a particular protein cd24's role in chemo resistance, and I have found out with MTT assay that shCD24 cells shows more death than wild type cancer cells for 2 of the...
16 July 2019 8,791 3 View
Basically the BSE has low resolution than the Secondary Electrons (SE). But totally SEM including FE-SEM resulting in low vertical resolution, Why?
26 May 2018 476 5 View
Functionalising Graphene with antibody to carry out ELISA.. What are the various methods to link antibody?
24 August 2013 4,615 5 View
I am using a BIAM (N-(Biotinoyl)-N'-(Iodoacetyl)Ethylenediamine) probe to label reactive cysteine residues. I target to identify the labeling sites by LC MS/MS analysis. But the challenging part...
15 January 2013 8,636 2 View
The " COVID-19 " pandemic has been an unprecedented situation with rapid research and development taking place. There has been a lot of data that is being generated like the total number of...
01 January 1970 1,498 5 View
I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...
14 February 2021 8,699 3 View
I performed in-silico acetylation using PyTM plugin but there are certain clashes that I want to resolve. I couldn't perform energy minimization using gromacs, chimera as they do not have default...
26 September 2020 3,907 6 View
I am converting maleic acid into maleic anhydride via dehydration in xylene and I would like to determine the impurities in the product. spcifically maleic/fumaric acid content and xylene. as well...
31 May 2020 1,109 6 View
In recent experiments acetylating cellulose fibers extracted from the leaves of a particular plant, weight loss was experienced. Experiments were repeated several times and reaction mixture was...
18 March 2020 4,599 3 View
I am trying to detect changes in Histone PTMs with or without treatment in nuclear extracts from cell lysates. I use a Hypotonic buffer (20 mM Tris pH 7.4, 10mM NaCl, 1mM MgCl2, 0.5mM EDTA)...
10 March 2020 2,626 6 View
acid can be converted into anhydride by sublimatiom?
05 March 2020 8,313 2 View
synthesis of poly (styrene-alt-maleic anhydride) copolymer with actual procedure
09 October 2019 5,590 1 View
Hi all, I am planing to perform a MD simulation of a protein with two acetylated lysines. Which workflow do you recommend me to follow? Has anyone used Vienna-PTM 2.0? any preferred force...
06 July 2019 8,471 2 View
It is nitration reaction and using the reagents such as nitric acid, acetic acid or acetic anhydride and sulphuric acid
06 July 2019 5,331 8 View
Average surface energy in the unit of mJ/m2 .
12 June 2019 1,854 2 View