How to separate protein using Biotynylated Streptavidin method? Is there any alternative procedure?
what is the reason behind this?is it due to the cDNA containation? usually how much cycles we are considering for doing PCR ?
06 December 2023 1,168 0 View
I ve run AGE for cDNA but i got like this.what is the reason behind this?
13 November 2023 198 5 View
what is the normal range in case of cell lines?
28 October 2023 3,309 3 View
How long we have to trypsinize A549 Cell as well as Beas2b cell line?? is it due to to fault during cryopreservation ?
28 October 2023 6,894 1 View
Is that ok to use DMEM media for maintaining beas2b cell line?
28 October 2023 5,870 3 View
Does cell lysis affect RNA isolation? I ve done my cell lysis by vortexing after that i ve done RT but im not geting the results.
28 October 2023 7,081 1 View
As a control i thought to use A549 Cell line. which is an alveolar epithelial cell line.Is that possible to use another cell line like mcf or normal lung epithelium as control group? why ?
24 September 2023 6,153 2 View
Greetings, I have perfomed DLS of my xerogels but the pdi >1 , between 1.2-2 and standard deviation is very large (300-700) . Can I interpret my result with this data or I should repeat.
08 August 2023 6,734 2 View
Method to identify NETosis
27 June 2023 6,536 3 View
I am trying to do amine protection of glycine amino acid using Di-tert-butyl-dicarbonate. Can someone suggest me the right reaction conditions for the same. Thank you
17 February 2023 8,505 3 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
What is the best way to obtain high-performance aptamers after performing sequence amplification by asymmetric PCR other than biotin and streptavidin?
17 April 2024 6,973 2 View
Does avidin binds to streptavidin?
14 April 2024 5,692 1 View
I'm having a problem with intense background noise in immunofluorescence for my biotinylated protein of interest conjugated with streptavidin (attached with a fluorophore) in mouse liver tissue...
11 April 2024 6,395 0 View
I have a question for the professionals. The essence of the problem: I fix the oligos on a plastic substrate, they play role of primers in solid-phase PCR. I carry out a one-step PCR with...
28 March 2024 9,110 2 View
Hi, I want to study RNA interacting with my test compound I am planning to do a RNA- pulldown experiment using streptavidin magnetic beads and biotin conjugated compound. I will detect...
22 February 2024 8,684 1 View
I have labeled my replicating bacterial DNA with 5-Ethynyl-2'-deoxyuridine (EdU) and want to click it with biotin-PEG-3-azide followed by enrichment with magnetic streptavidin beads. So far, I...
06 February 2024 5,381 0 View
Hi, I want to perform chromatography on viruses which are larger than the protein targets people typically use chromatography columns on. Because of this I need to use 2% agarose beads which...
30 October 2023 2,771 3 View
Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...
16 October 2023 3,778 2 View
My peptide has biotin attached to it, and I am trying to bind that to streptavidin agarose beads. After 1-4 hrs of mixing, when washing with 5X DPBS, almost all the biotin-peptide comes off. Are...
02 October 2023 1,559 0 View