Which one should be the control point and slave node in assigning MPC constraint using ABAQUS?

Hello everyone, I am modeling a bridge with and orthotropic deck, floor beams, edge beams and main girders. I have to connect bridge deck with the floor beams and floor beams with the main...

22 November 2020 5,750 4 View

What is the best suited element type for meshing the notched parts of structural steel members?

Hello every one, I am doing finite element analysis of a notched steel component with nonlinear material properties. I have tried different element types for meshing and the analysis results are...

11 October 2020 5,433 3 View

How the optical property will modify based on penn energy?

For single crystal

18 September 2017 1,650 1 View

Can you send a code for tweet segmentation?

12 April 2017 8,236 2 View

How do we exactly analyze ELISA results obtained from ELISA reader and draw conclusions from it?

Calculation of results from ELISA data

04 June 2012 1,825 4 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

How to bind streptavidin to the surface of acrylamide?

We are fabricating acrylamide beads as probe substrate. How can we make streptavidin bind to the surface of the acrylamide? Thanks!

09 December 2020 8,141 7 View

mScarlet Elisa Detection?

I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...

20 September 2020 730 2 View

Best way to add streptavidin onto flat surface?

I will be adding streptavidin onto the surface of a graphene coated glass slide; thereafter, I will be analyzing with a MALDI-TOF. I was wondering the best way to go about this? I was thinking of...

07 September 2020 5,902 4 View

How can I break biotin-streptavidin bond and separate them?

I am working with a florescent compound that is biotinylated. When strepdavidin is present in the system, the binding of the protein with the compound quenches the florescence. I need to perform...

16 August 2020 6,937 8 View

Help with gold nanoparticle with nile blue, why sodium azide???

Hello! I have been trying to understand this for more than a year now, and I am at a loss. Can anyone help, please?? So, I follow this protocol :Statistical Correlation Between SERS Intensity...

31 May 2020 658 10 View

Do you have any suggestions to synthesis of positive gold nanoparticles with streptavidin on its surface?

I want to synthesize positively charged gold nanoparticles and then surface decorate with streptavidin. The ideal size would be 20nm or less. Please help.

18 March 2020 7,955 2 View

SAC Well Coating?

The wells are polystyrene coated with streptavidin via a biotin BSA linking layer using the SAC well coating method. What is SAC coating method?

06 March 2020 3,016 3 View

Anomaly in biotin tagged peptide. What could be the reason?

I have 1Kd biotin tagged peptide. The alone peptide is running at the expected size but when I am allowing it to bind to streptavidin beads it's running around 10Kd. What could be the reason?

02 March 2020 1,681 3 View

Why is MACS positive selection not working?

Hi, everyone! I'm trying to select HEK293FS cells expressing SBP-LNGFR protein. The protein presence on the cell membrane is confirmed with immunofluorescence. But the MACS procedure with...

03 February 2020 5,791 4 View