Hi, I want to perform chromatography on viruses which are larger than the protein targets people typically use chromatography columns on. Because of this I need to use 2% agarose beads which have the largest pore size, however this is a niche use case so I cannot find an off the shelf solution.

I cannot find a protocol on the web. Would a standard EDC-NHS conjugation approach be suitable? Are any linker molecules needed? Are there better chemistries that target specific moieties on streptavidin to better orient it on the bead? I have done some bead conjugation work before, I only require a protocol that outlines linkers, chemistry etc. Or can anyone point me to a supplier. Thank you.