I'm having problem with intense background noise in immunofluorescence for my protein of interest conjugated with streptavidin in mouse liver?

12 April 2024 0 6K Report

I'm having a problem with intense background noise in immunofluorescence for my biotinylated protein of interest conjugated with streptavidin (attached with a fluorophore) in mouse liver tissue (parafilm fixed/ frozen). This doesn't happen with bile duct tissue. I think the biotin or streptavidin is reacting with some liver-specific stuff, hence such a huge background.

Does anyone have any similar experience or a solution for this?

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