Can anyone suggest methods for GEnomic DNA isolation from fungus ( ustilago and Aspergillus)?

01 January 2018 1 9K Report

Im looking for best fungal genomic DNA isolation method.And isolated DNA needs to subject PCR reaction.Therefore can anyone assist me protocols

Katie A Burnette

I've used a DNeasy Blood & tissue kit. Works great with fungi.

Badges
Science topic

More Deepak James's questions See All

I want full genome sequence of A.terreus which could produce Itaconic acid .Can anyone assist me,where i get this information?

Hi to all good ones, I want full genome sequence of A.terreus which could produce Itaconic acid .Can anyone assist me,where i get this information?

03 April 2018 1,927 4 View

I want full genome sequence of A.terreus which could produce Itaconic acid .Can anyone assist me,where i get this information?

Hi to all good ones, I want full genome sequence of A.terreus which could produce Itaconic acid .Can anyone assist me,where i get this information?

03 April 2018 4,276 3 View

Why i am facing Issue in growing Synechocystis sp. ATCC 27184?

I am Trying to grow Synechocystis ATCC 27184 using BG-11 broth. Also providing ligth source 2000-3000 lux using 40Watt LED bulb as light souce. Incubated at 26°C for 1 to 2 weeks and zero...

03 April 2018 496 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View