You can use Millipore's Cresendo western HRP substrate. Its the best one i have used.

Try this ECL kit from Biorad.

http://www.bio-rad.com/en-au/product/clarity-western-ecl-blotting-substrate.

Make sure you block your membrane well to eliminate as much background as possible. 

Good luck!

Thanks for all your sugestions and I will try and see.

@Ebr Bio

In case you are still screening products, here are some other options:

• Economical performance

https://www.licor.com/bio/products/reagents/westernSure_Chemi_Reagents/chemi_substrates/ecl.html

Representative publications:

https://www.researchgate.net/profile/Jian_Zhang10/publication/284232296_Mono-2-ethylhexyl_phthalate_induces_apoptosis_through_miR-16_in_human_first_trimester_placental_cell_line_HTR-8SVneo/links/5668351f08ae34c89a05ac2f.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604159/

• Longest duration of signal

https://www.licor.com/bio/products/reagents/westernSure_Chemi_Reagents/chemi_substrates/premium.html

Representative publications:

https://www.researchgate.net/profile/Olga_Grove/publication/272520517_Expression_of_Adiponectin_and_Its_Receptors_Is_Altered_in_Epithelial_Ovarian_Tumors_and_Ascites-Derived_Ovarian_Cancer_Cell_Lines/links/55c8a43508aeca747d66fb03.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894949/

• For pricing and promotions, please contact your local distributor:

https://www.licor.com/bio/distributors/

Hope this helps.

Article Mono-(2-ethylhexyl) phthalate induces apoptosis through miR-...

Article Expression of Adiponectin and Its Receptors Is Altered in Ep...