Which Crispr vector is suitable for Lactobacillus delbrueckii subsp. bulgaricus?

11 November 2015 1 6K Report

Dear all,

I am planing to do some genome editing with CRISPR/Cas9 in Lactobacillus delbrueckii subsp. bulgaricus (ATCC11842), but I am not sure which CRISPR/Cas9 is suitable for this bacteria strain? Does anybody has experience with this?

Thanks!

David Lubkowicz

Hey, take a look at the attached Link. They used CRISPR for genome editing in lactobacillus reuteri. Basically it works like in any other organism, where you determine your target with the appropriate PAM sequence. The most important thing is to choose the correct replicon and promoter that will work in Lactobacillus delbrueckii subsp. bulgaricus (ATCC11842). What I mean is, that it is not important which CRISPR you choose but the correct and stable expression.

Good luck

http://www.ncbi.nlm.nih.gov/pubmed/25074379

Badges
Science topic

Similar topics
Vectorization

More Ebr Bio's questions See All

Can anyone suggest me with ECL product?

Dear Al, I am working on WB, and looking for ECL products with lower background and stronger signal. I have tried the GE-ECL primer but it turned out to be not ideal. can anyone suggest me some...

04 May 2016 9,944 4 View

ICan anyone help with gG labelling with HRP protocol without colum seperation?

Dear all, I want to label IgG with HRP, the following protocol have to seperate labelled antibody with colum, which may cause sample lose. Does anyone have procols avoiding colum seperation steps...

06 July 2015 7,590 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View