I am currently working on N2A cells, but they are not very good candidates for confocal imaging. I was wondering if there are references on better suited neuronal cell lines for poly glutamine studies which can be better than N2A for imaging?
Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...
27 June 2024 1,221 1 View
I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.
23 June 2024 3,446 1 View
Hi all, We performed Yeast-Cell-Glucose-Uptake assay to estimate the antidiabetic potential of the test compound. The Metformin treated yeast cells, resulted in taking up more glucse than normal...
02 April 2024 7,657 2 View
pursuing phd on geospatial data security.suggest good paper on robust invisible blind watermarking for copyright protection
12 March 2024 4,917 2 View
in a community area, there is an multi microgrid systems, why we call it as multi microgrid system but not treated as single microgrid system. and difference between single microgrid and multi...
03 March 2024 9,306 1 View
I am facing an issue of wire bonding, trying to ball bond Au wire onto a metal stack/semiconductor (Au/Ti/GaN). Thickness of Au = 200 nm, Thickness of Ti = 20 nm. I observe that the metal...
07 January 2024 4,813 1 View
I am studying precision for my assay/kit with the following parameters followed: Panel members to be tested: 1. Weak positive 2. Medium positive 3. Strong positive 4. Negative All these were...
11 December 2023 1,812 1 View
how can i add all my articles and journals in research gate including those published in research gate?
22 November 2023 5,926 3 View
Atorvastatin used to treat Atherosclerosis, seems to causes hepatoxicity upon prolonged usage. So, to inhibit such problem we have introduced an natural substances which is reported for the...
09 November 2023 587 0 View
I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?
09 November 2023 8,266 2 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View