Can anyone suggest me a way to maintain hypoxia in yeast with approximately < 5ppm oxygen level ?

More Bijoy Mukut Buragohain's questions See All

Could you please recommend reputable researchers or research journals that delve into scram jet engines with air throttling?

The focus should be on examining kerosene fluid as the inlet fluid and placing significance on determining the specific value of carbon dioxide in this context.

19 January 2024 6,031 0 View

Are there individuals interested in proposing innovative omni directional wind turbine designs and collaborating on projects in this field?

Are there any researcher who have a desire or willingness to come up with creative and original ideas for designing wind turbines that can capture wind energy from all direction. The focus is on...

14 September 2023 409 0 View

How can I get access to tool ,Metacogn knowledge and Skills Assessment Inventory(MSA) developed by Annemie Desoete and colleague(2001a)?

My research aims at enhancing metacognition. For this purpose, I would like to use MSA for assess metacognition, however, I have not been able to find it.

13 July 2023 6,365 0 View

Is it possible to devise innovative techniques that surpass the limitations imposed by the Carnot cycle?

By pushing the boundaries of conventional thermodynamic cycles, exploring new materials, and embracing sustainable technologies, I, as a researcher, can aim to enhance the efficiency of energy...

11 June 2023 5,275 4 View

Which body of water, the one in the bowl or the one in the glass, will heat up more rapidly?

In a controlled experiment, if a bowl of water is placed on a gas stove to boil and a glass is submerged within that bowl, assuming that the water temperature in both the bowl and the glass is...

11 June 2023 10,029 1 View

Is it feasible to utilize small wind turbines to power large turbines? What would be the impact on efficiency - an increase or decrease?

Is it technically viable to employ small wind turbines to generate electricity that could, in turn, power larger turbines? If such a setup were implemented, what would be the effect on overall...

11 June 2023 5,291 3 View

Could someone suggest a researcher who has prior experience working with Supercritical CO2 power cycles or in the field of gas power cycles?

I am looking for suggestions from researchers who possess previous experience or specialized knowledge in Supercritical CO2 power cycles or have conducted extensive research in the field of gas...

09 June 2023 8,539 0 View

Could someone clearly specify the thermal and mechanical loads experienced by composite materials under Hypersonic conditions?

Hypersonic vehicles, which travel at speeds greater than Mach 5, experience extreme thermal and mechanical loads during flight. The objective of this research is to investigate the behavior of...

08 June 2023 8,727 1 View

Can it be stated that severe boundary layer separation results in increased turbulence?

Can it be posited that the presence of a severe boundary layer separation, characterized by the detachment of the fluid flow from a solid surface, serves as a significant contributing factor to...

03 June 2023 7,334 23 View

What factors govern the formation and dynamics of turbulent flow, and how can we better understand and predict its behavior?

This question delves into the fundamental nature of turbulence, a ubiquitous phenomenon in fluid dynamics that is characterized by chaotic and unpredictable fluid motion. Exploring the mechanisms...

01 June 2023 334 6 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Why my yeast colony PCR gel has one specific band , but the size is incorrect even when the plasmid DNA that is originally transformed is correct?

The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...

01 July 2024 2,356 6 View

Is measurement of oxygen uptake rate dependent on the DO concentration in the sample?

One problem w the BOD bottle type of OUR measurement is that it can only be used in a system that is not DO limited (typically assumed about 2 mg/l or more). If the system is running at low DO and...

01 July 2024 1,282 2 View

Ritam Hazarika

Hypoxia and Fungal Pathogenesis: To Air or Not To Air?

Nora Grahl, Kelly M. Shepardson, [...], and Robert A. Cramer

Daniel Brady Mills

If you can plug the flask with a rubber stopper with a glass tube inserted through it, you can introduce N2 into the system to remove the O2 in the solution to reach