Bijoy Mukut Buragohain

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Questions related from Bijoy Mukut Buragohain

Does Triton x-100 in lysis buffer effects the binding of His tagged proteins in the Ni- NTA beads ?

Triton X 100 is used to increase the solubility of the protein when used in the lysis buffer for bacterial lysis. But I am not sure how much percentage can be used so that it increases the...

26 December 2022 5,839 3 View

Can anyone suggest me a way to maintain hypoxia in yeast with approximately < 5ppm oxygen level ?

I am trying to grow approximately 50ml of yeast culture in an Erlenmeyer flask with shaking in hypoxic conditions with < 5ppm oxygen. In hypoxic chambers, the oxygen level is always around...

27 July 2021 2,202 2 View