Hi,
Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound,
I've observed 2 peaks in DCF-DA in WT. It's normal? In some experiments, the second peak appears always but variable in intensity. Do normal lymphocytes have a different basal ROS corresponding to different lymphocytes subsets?
the second question in my case, how can I interpreter my data? 2T treatments produce or not rise of ROS? should I normalize my gMFI to cell numbers?
All works report only one peak of ROS, that's for me it's strange
Ps the different count is due to loss of some lymphocyte by treatments.
An experienced immunologist maybe could me help.
Article Redox Environment in Stem and Differentiated Cells: A Quanti...
The above paper may give you a hint. You could explore PubMed or another literature search to find out what you want.
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