Francesco De Leonardis

6 Questions 3 Answers 0 Followers

Questions related from Francesco De Leonardis

Can anyone suggest me a sorting buffer for H9C2 cardiomyocytes ?

Can anyone suggest me a pre-sorting and post-sorting buffer for H9C2 cardiomyocytes cell lines? I'm using BD Facs Melody Thanx

30 November 2022 6,852 0 View

Granzyme B detection in activated CD3/CD28 on CD8 +, how long stimulate?

Hi, I want to detect Granzyme B after CD8+ stimulation by Dynabeads. My question is : Ho long should I incubate my Cd8+ with beads to detect (flow cytometry) GzB? and when should I incubate cells...

04 February 2022 1,818 2 View

Basal ROS in Lympohocytes. Why I observe two peaks in the control?

Hi, Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound, I've observed 2 peaks in DCFH-DA in WT. It's normal? In some experiments, the second peak...

22 December 2021 6,423 1 View

Lymphocytes ROS, Why I observe 2 peak in Control Lymphocytes?

Hi, Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound, I've observed 2 peaks in DCF-DA in WT. It's normal? In some experiments, the second peak...

19 December 2021 4,052 1 View

Why I'm still having negative gfp cells after sorting?

Why I still having negative gfp mioblast sorted after gating on positive gfp over 10^4 signal? Fig1 negative population fig 2 pre sorting fig 3 post sorting. Facs melody nozzle 100 um no aggregates..

16 November 2021 5,001 3 View

Does anyone have experience with MCL-1 or BCL-2 detection in resting lymphocytes by flow cytometry?

Does anyone have experience with MCL-1 detection in resting lymphocytes by flow cytometry? I've tried to stimulate peripheral lymphocytes with Il-7 10 ng/ml for 6 hours and with CD3/CD28 beads for...

01 January 1970 5,103 0 View