Francesco De Leonardis

4 Questions 2 Answers 0 Followers

Questions related from Francesco De Leonardis

Can anyone suggest me a sorting buffer for H9C2 cardiomyocytes ?

Can anyone suggest me a pre-sorting and post-sorting buffer for H9C2 cardiomyocytes cell lines? I'm using BD Facs Melody Thanx

30 November 2022 6,815 0 View

Granzyme B detection in activated CD3/CD28 on CD8 +, how long stimulate?

Hi, I want to detect Granzyme B after CD8+ stimulation by Dynabeads. My question is : Ho long should I incubate my Cd8+ with beads to detect (flow cytometry) GzB? and when should I incubate cells...

04 February 2022 1,811 2 View

Basal ROS in Lympohocytes. Why I observe two peaks in the control?

Hi, Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound, I've observed 2 peaks in DCFH-DA in WT. It's normal? In some experiments, the second peak...

22 December 2021 6,363 1 View

Lymphocytes ROS, Why I observe 2 peak in Control Lymphocytes?

Hi, Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound, I've observed 2 peaks in DCF-DA in WT. It's normal? In some experiments, the second peak...

19 December 2021 4,048 1 View