Hi,
Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound,
I've observed 2 peaks in DCFH-DA in WT. It's normal? In some experiments, the second peak appears always but variable in intensity. Do normal lymphocytes have a different basal ROS corresponding to different lymphocytes subsets?
the second question in my case, how can I interpreter my data? 2T treatments produce or not rise of ROS? should I normalize my gMFI to cell numbers?
All works report only one peak of ROS, that's for me it's strange
Ps the different count is due to loss of some lymphocyte by treatments.
thanx for suggestions
https://www.researchgate.net/post/Why_my_reactive_oxygen_species_ROS_assay_using_H2DCFDA_revealed_double_shifted_peaks
https://www.researchgate.net/post/ROS_determination_by_FACS
The above RS discussion may be helpful. Good luck.
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