I am to do immunohistochemistry on peripheral blood film to demonstrate CD13 and CD33 markers qualitatively/quantitatively. Therefore, seeking for your help in that respect, please.
This has nothing to do with immunohistochemistry per se but immunocytochemistry may be better related.
Check any protocol online, they must have done it; talk to google, I am sure you shall be well answered.
Better still, see the datasheet of your antibodies.
Rather, you may contact the reagent vendour of your choice for better communication, every protocol exists for advance immuno-tech.
Again and finally, I think Saleh Alkarim could be of help.
Dear Danladi
It would have been useful to know the purpose of this work. If it is for clinical diagnostics, flow cytometry remains the 'gold standard' technique because it is sensitive, reproducible and minimises subjective errors. However, if you have no access to flow cytometry, you could consider immunocytochemistry (eg APAP technique). Remember, this technique is at best semiquantitative. If the purpose is basic scientific research wanting to visualise location and distribution of the antigen molecules, confocal microscopy would be very useful.
You may consider doing ELISA. Realtime PCR may be an option as well to see expression level at RNA.
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