I am trying to arrest MCF 7 cells at S phase following which I will perform Knock down experiments. A detailed protocol for the arrest and subsequently how to carry on the siRNA mediated knockdown would be helpful.
Hello
kindly look at links here may help you
Good Luck
https://link.springer.com/protocol/10.1385/1-59259-889-7%3A033
https://worldwidescience.org/topicpages/c/cell+cycle+synchronization.html
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-333
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