S Paul Chowdhuri

7 Questions 4 Answers 0 Followers

Questions related from S Paul Chowdhuri

Can someone suggest a protocol for arresting cells at late G2 phase without using RO3306?

Actually I am trying to study a protein which is phosphorylated by CDK1. So I cannot use RO3306 but I need to arrest cells at late G2 followed by temporally controlled release into the subsequent...

28 November 2020 3,530 4 View

Can any one suggest an invitro kinase assay protocol when one does not have purified/recombinant proteins availabe (both kinase and the substrate)?

I do not have purified or recombinant kinase or the substrate proteins of my interest. So I transfect cells ((HEK293)/MCF7) with tagged constructs FLAG-tagged for substrate of interest and...

21 March 2018 8,771 2 View

Can anyone suggest Aphidicolin mediated cell synchronisation protofol for S phase arrest in MCF7 cells?

I am trying to arrest MCF 7 cells at S phase following which I will perform Knock down experiments. A detailed protocol for the arrest and subsequently how to carry on the siRNA mediated knockdown...

15 April 2017 6,381 1 View

Can anyone suggest a detailed protocol for alkaline COMET Assay for Leishmania donovani?

Please suggest what drug can be used as a positive control in case of Ld

29 November 2016 4,668 0 View

Why despite being a 646 amino acid residue protein, human Wee1 has a molecular weight of approx 95 kDa and 72 kDa? antibodies of both sizes available?

which antibody should be used

08 August 2016 6,906 1 View

Has anyone generated a K/O using pSpCas9(BB)-2A-GFP (PX458) from Addgene? It would be really helpful if the experience is shared?

MCF7 is the cell line

15 March 2016 1,362 2 View

I am trying to knockdown a particular gene with CRISPR/Cas9. Can anybody kindly suggest me in detail how to design the oligonucleotide for my gRNA?

Help me with the gRNA design softwares and also how do I be sure which to choose from the list suggested by the software. I hae been trying tho use the Xhang Lab'sprotocol

09 February 2016 6,301 3 View