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Actually I am trying to study a protein which is phosphorylated by CDK1. So I cannot use RO3306 but I need to arrest cells at late G2 followed by temporally controlled release into the subsequent...
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I do not have purified or recombinant kinase or the substrate proteins of my interest. So I transfect cells ((HEK293)/MCF7) with tagged constructs FLAG-tagged for substrate of interest and...
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