Ethanol or triton x100 at 2% can be used to disolve cholesterol,but if it is non toxic in your systeem i am not sure

Have you tried CHCl3?

Any non-toxic liophilic (i.e. liquid oil) solvent may be used. There are no true solutions of cholesterol  in aqueous media, only colloids and suspensions may be formed.

I dissolve cholesterol standard (for HPLC analyses) in propanol -2-ol 1% in n-hexane.

We use dichloromethane.

One can use low percentage ethanol (less than 5%) for dissolving  cholesterol  

Choline based ionic liquid can be used.

cholesterol dissolve in solvent like chloroform, dichloromethane, ethanol, DMF, DMSO.

I use Dichloromethane too

Grasso, thanks for your response. Dichloromethane  can used a solvent in animal studies? 

what do you mean with animal studies? I use it with turkey meat.

Can I feed the animal (rodent) cholesterol by dissolving in Dichloromethane? Is it toxic?

You can dissove cholesterol in ethanol or aceton

Cholesterol dissolving solvents are  ethanol, methanol, DMF, Ethyl  acetate, Chloroform ,acetonitrile  ether etc.

 what is it limit for ethanol or methanol?

if we are going to use less than 5% ethanol how much or percentage of cholesterol can be dissolved in this solvent?

Moderately soluble in hot alcohol; soluble in benzene, methanol, oils, fats and aqueous solution of bile salts.

Its water solubility is 0.095 mg/L (at 30 °C)

National Center for Biotechnology Information. PubChem Compound Database; CID=5997, https://pubchem.ncbi.nlm.nih.gov/compound/5997 (accessed Feb. 3, 2017).

it dissolved totally in chloroform

How can I aliquot the cholesterol after dissolve it in chloroform? I mean what kind of tubes should I use?

See the answers (including mine) in the other discussion thread:

https://www.researchgate.net/post/Can_anybody_tell_me_which_solvent_can_dissolve_beta-sitosterol