and read about that possibility? (Guessing there exuist some other methods too....)
Classical Histochemistry stains on FFPE-Formaldehyder-Fixed-Paraffin-Embedded material might fail because of elution of the cholesterol stuff during (fixation too, but most of it during) dehydration in higher alcoholsand intermediate. Best of Luck!
Article A novel cholesterol stain reveals early neuronal cholesterol...