I am working on the conjugation of antibodies on gold nanoparticles surface recently and I was looking for an optimized method for this purpose based on EDC/NHS. Given the many methods available, I would be grateful if anyone could suggest an optimized protocol with high efficiency.
Dear Mehrdad Ameri, data are extremely wide to select a given approach. It is up to you to follow the one that suits your work conditions. Please check the following documents. My Regards
https://www.researchgate.net/post/Synthesis_of_gold_nanoparticle-antibody_conjugate_using_EDC_sulfo-NHS
https://doi.org/10.1016/j.sbsr.2016.04.002
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559877/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761283/
https://www.nature.com/articles/s41598-017-12677-w
Edc/nhs is a pain in the ass. There is no optimized protocol. Hydrolysis is the enemy. My recommendation is to not use it or use huge amounts of both with respect to carboxylic groups.
EDC/NHS chemistry is not very reliable and not an obvious method to conjugate antibodies to plain gold nanoparticles. On fresh gold particles, antibodies might be adsorbed directly or via a thiol group either obtained by partial reduction or a thiolation reagent.
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