Can anyone suggest a simple protocol for analysing protein aggregates under scanning electron microscope. I need details of sample preparation?

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Hediye Erdjument-Bromage

Hi Arun,

Have you tried using detergent at 0.1% (v/v)? A couple of microliters may break the aggregates.

Best,

Hediye.

Arun Upadhyay

Not yet.. Actually I want to study the interactions of small molecules with aggregates. Can you suggest how should I prepare my samples for scanning electron microscope analysis. Please.

Dear Arun,

In that case, detergent may not be a good choice. At least, it has to be a very mild detergent.

I have colleagues who are in the SEM field. Let me ask and get back to you.

Yeah Sure Hediye....Thanks for concern. Waiting for ur suggestions.